you obtained a count of 35 cells from one box with a 5-fold (1:5 or technically 1:4) dilution from a tube of 5ml of cell suspension

a) concentration of cells

b) total number of cells in tube

c)volume needed for 2x10^6 cells

Answers

Answer 1

a) To calculate the concentration of cells in the original cell suspension, we can use the dilution factor. In this case, the dilution factor is 1:5, which means that 1 part of the original cell suspension was diluted with 4 parts of diluent.

Since you obtained a count of 35 cells from the diluted sample, we can multiply this count by the dilution factor to determine the concentration of cells in the original cell suspension:

Concentration = (Count / Dilution factor) = (35 cells / 1:5 dilution) = 35 cells * 5 = 175 cells per unit volume

b) The total number of cells in the tube can be calculated by multiplying the concentration of cells by the volume of the cell suspension. In this case, the volume of the cell suspension is 5 mL.

Total number of cells = Concentration * Volume = 175 cells/mL * 5 mL = 875 cells

c) To calculate the volume needed for a specific number of cells, we can rearrange the formula for concentration:

Volume = (Number of cells) / (Concentration)

In this case, you need 2x10^6 cells. Plugging the values into the formula:

Volume = (2x10^6 cells) / (175 cells/mL) = 11,428.57 mL

Therefore, you would need approximately 11,428.57 mL or 11.43 L of the cell suspension to obtain a total of 2x10^6 cells.

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Related Questions

What is the number of different species of plants and animals in an area

Answers

Answer:

Biodiversity

Explanation:

Biodiversity is a term used to describe the enormous variety of life on Earth. It can be used more specifically to refer to all of the species in one region or ecosystem. Biodiversity refers to every living thing, including plants, bacteria, animals, and humans.

What is the ultimate source of all the energy that powers your cells ( ... all the energy that powers your cells (and > 99.9% of all life on earth)?

Answers

The ultimate source of energy that powers your cells and most > 99.9% of all life on earth is sunlight.

Energy into each food chain is brought by plants or algae. Plants have the capability to produce their own food through the process of photosynthesis.

In the process of photosynthesis, light energy from the sun is used to convert water and carbon dioxide into glucose and oxygen. It is through the process of photosynthesis that plants produce their own energy. All other life forms depend on plants in a way because each food chain either begins with a plant or algae. This energy from photosynthesis is then transported through each trophic level. Hence, the ultimate energy source for our cells is the light energy from the sun.

As the process of photosynthesis requires light energy from the sun, hence sunlight is the source of energy that powers our cells and of all life on earth.

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Both plant and animal cells contain -
A. mitochondria, cell membrane, and chloroplasts.
B. a cell membrane, cytoplasm, and nucleus.
C a cell membrane, cell wall, and nucleus.
D. mitochondria, a cell wall, and chloroplasts.

Answers

A cell membrane cell wall and nucleus. I think

Both plant and animal cells  contain a cell membrane, cytoplasm and nucleus because animal cell does not contain cell wall and plant cell have cell wall but both of the cell contain cell membrane, cytoplam and nucleus.Thus option B is correct.

What is the difference between plant and animal cell?

Plant cell consist cell wall but in animal cell, cell wall is absent. Nucleus is present in both of the cell. animal cell does not contain cell wall and plant cell have cell wall but both of the cell contain cell membrane, cytoplam and nucleus.

The main function of vacuoles present in plant cell as it help in storage of waste materials in a plant cell and maintain a proper pressure in a plant cell.

Both plant and animal cells  contain a cell membrane, cytoplasm and nucleus because animal cell does not cotain cell wall and plant cell have cell wall but both of the cell contain cell membrane, cytoplam and nucleus.Thus option B is correct.

There are various differences are found in plant and animal cell. The very first difference is shape of the cell, plant cell is square or rectangular in shape but animal shape is irregular in shape.

Plant cell consist cell wall but in animal cell, cell wall is absent. Nucleus is present in both of the cell.

The main function of vacuoles present in plant cell as it help in storage of waste materials in a plant cell and maintain a proper pressure in a plant cell.

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what factor determines how a post-synaptic/effector cell will respond to the presence of a particular pre-synaptic neurotransmitter

Answers

When neurotransmitter molecules bind to the receptors, a synaptic impact is produced. The impact is proportional to the neurotransmitter's average concentration.

The following are some of the elements that affect how well neurotransmitters attach to their receptors: the neurotransmitter concentration. the relationship between the receptor and the neurotransmitter. The type of channel that is linked to the receptor and the concentration of permeant ions both within and outside the cell determine whether a postsynaptic response is an EPSP or an IPSP.

Depolarization, or a reduction in negative charge, forms an excitatory PSP because it can stimulate the production of a nerve impulse if the neuron achieves the crucial threshold potential (action potential).

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The factor that determines how the post-synaptic/effector cell responds to the presence of a particular pre-synaptic neurotransmitter is: the net change in post-synaptic membrane voltage.

A neurotransmitter is a chemical substance that acts in between two nerve cells to transmit the electrical signals in chemical forms from one nerve cell to another. Neurotransmitter can be excitatory or inhibitory. The example of neurotransmitters are: acetylcholine, dopamine, GABA, glutamate, etc.

Membrane voltage, also called membrane potential is defines as the difference in the charge between the inside and the outside of the membrane. This difference in charge is due to the different concentrations of ions on both sides.

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what is one criticism of the current theories that attempt to explain sexual orientation?

Answers

One criticism of the current theories attempting to explain sexual orientation is that they often oversimplify a complex and multifaceted phenomenon.

Sexual orientation is influenced by a combination of genetic, hormonal, environmental, and sociocultural factors, and it is not yet fully understood. Some critics argue that existing theories tend to focus on a single factor or a limited set of factors, such as genetics or childhood experiences, without adequately considering the interplay and complexity of these influences. Furthermore, there is ongoing debate about the extent to which sexual orientation is innate or shaped by environmental factors, and current theories may not fully capture the dynamic nature of sexual orientation development. As research continues, it is important to critically examine and refine existing theories to better reflect the complexity and diversity of human sexuality.

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predict what will occur in your body after a vigorous run. Explain the chemical process that supports your prediction.

Answers

Answer:

More blood is pumped to the exercising muscles to deliver that additional O. Without enough oxygen, lactic acid will form instead. Lactic acid is typically flushed from the body within 30 to 60 minutes after finishing up a workout.

Explanation:

I hope this helped ;)

A calico cat shows both the traits for orange fur and black fur.
What kind of allele expression is this?

A calico cat shows both the traits for orange fur and black fur.What kind of allele expression is this?

Answers

Codominance

Explanation: Becuase both of the traits are showing.

The allele expression in the case of a calico cat which shows both the trait for orange fur and black fur is Codominance.

Codominance is a hereditary model that states that in the heterozygous there is no recessive allele, but on the contrary, both alleles behave as dominant and both manifest themselves without mixing.

Codominance is an important aspect of the crossing process because the two allele genes are expressed, but not joined. An example of this is calico cats that have two X chromosomes in each of their cells.

In calico cats, the gene for the orange fur is located on the X chromosome and they have one allele for the black fur. The only way that both alleles occur together and combined with white is that there are two X chromosomes and these are codominant or these express at the same time.

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A calico cat shows both the traits for orange fur and black fur.What kind of allele expression is this?

WILL MARK BRAINIEST PLS HELP!
Using the traits and alleles from this chart, indicate the possible genotype(s) for the following plants:
22. Homozygous wrinkled ……………………………………………………………..
23. Homozygous round …………………………………………………………………..
24. Round heterozygous…………………………………………………………………..
25. Green hybrid…………………………………………………………………..
26. Heterozygous green pods…………………………………………………………………..
27. Yellow pods…………………………………………………………………..
28. Short stems…………………………………………………………………..
29. Tall stems…………………………………………………………………..
30. Yellow seeds…………………………………………………………………..
31. Pure tall stem ………………………………………………………………….
32. Homozygous tall stem…………………………………………………………………..
33. Heterozygous tall stem…………………………………………………………………..

WILL MARK BRAINIEST PLS HELP!Using the traits and alleles from this chart, indicate the possible genotype(s)

Answers

Answer:

look below

Explanation:

22. rr x rr

23. RR x RR

24 Rr x Rr

25. ??

26. Gg x Gg

27. gg x gg

28. ll x ll

29. Ll x LL

30. YY x YY, Yy x Yy

31. LL x LL , Ll x Ll

32. LL x LL

33. Ll x Ll

The diagram below represents a reproductive process. The three
structures indicated by letter X are known as *
4 points
ova
Ο Ο
polar bodies
homologous chromosomes
O
O
embryos

The diagram below represents a reproductive process. The threestructures indicated by letter X are known

Answers

Answer:

Polar bodies is the correct answer

question 45 58) movement of the chromosomes during anaphase would be most affected by a drug that prevents a) nuclear envelope breakdown. b) cell wall formation. c) elongation of microtubules. d) shortening of microtubules. e) formation of a cleavage furrow.

Answers

The correct option is (d). During anaphase, movement of the chromosomes would be most affected by a drug that prevents shortening of microtubules. This is because it is the shortening of microtubules that pulls the chromosomes towards the poles of the cell in order to make the two sets of chromosomes separate.

During anaphase, movement of the chromosomes would be most affected by a drug that prevents shortening of microtubules. This is because it is the shortening of microtubules that pulls the chromosomes towards the poles of the cell in order to make the two sets of chromosomes separate. In this process, the sister chromatids are pulled apart, one towards each pole and the result of this separation is that each pole now has a complete set of chromosomes that will be used to form two identical nuclei. Chromosomes are structures in the nucleus of a cell that contain genetic material in the form of DNA. DNA is the genetic material that is passed on from parents to offspring and carries the information that determines the characteristics of the organism. Chromosomes are responsible for the transmission of this information from one generation to another. The nuclear envelope is the double-layered membrane that surrounds the nucleus of a cell. It separates the contents of the nucleus from the cytoplasm and regulates the flow of molecules in and out of the nucleus. The formation of the nuclear envelope is important for the proper functioning of the nucleus and its contents. Microtubules are a type of cytoskeleton filament that plays a key role in many cellular processes. They are involved in the movement of organelles and other structures within the cell, as well as in the division of the cell. During cell division, microtubules form the spindle fibers that attach to the chromosomes and help to pull them apart. Movement of chromosomes is a crucial part of cell division, and the process requires a complex interaction of different cellular components. The cytoskeleton plays a key role in this process, and microtubules are an essential component of the cytoskeleton that are involved in the separation of chromosomes during cell division. Microtubules are long, thin, tube-like structures that are made up of protein subunits. They are involved in many different cellular processes, including cell division, cell movement, and intracellular transport. During cell division, microtubules form the spindle fibers that attach to the chromosomes and help to pull them apart.The movement of chromosomes during cell division is a complex process that requires the coordinated action of many different components. Microtubules are involved in this process because they attach to the chromosomes and pull them towards the poles of the cell. This movement is driven by the shortening of the microtubules, which causes them to exert a pulling force on the chromosomes.The formation of a cleavage furrow is a process that occurs during cytokinesis, which is the final stage of cell division. During cytokinesis, the cell membrane pinches inwards and eventually divides the cell into two separate cells. The formation of the cleavage furrow is not directly involved in the movement of chromosomes during anaphase. Therefore, the correct answer to this question is (d) shortening of microtubules.

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the movement of substances from an area of high concentration to and low area is called

Answers

Answer:

Diffusion

Explanation:

Diffusion is a passive process of transport. A single substance tends to move from an area of high concentration to an area of low concentration until the concentration is equal across the space.

Answer: diffusion

Explanation:  

the action of spreading a light from a light source evenly so as to reduce glare and harsh shadows  

i hope thats right if it is have a nice day

Select all that occur with increased sympathetic
stimulation.
Check All That Apply
Release of insulin from the pancreasRelease of insulin from the
pancreas
Contraction of the gallbladder, resultin

Answers

Increased sympathetic stimulation results in the following events: Contraction of the gallbladder resulting in the release of bile into the digestive tract. Heart rate and blood pressure will increase. Blood flow to the digestive system will decrease. Blood flow to skeletal muscles will increase.

Dilation of the pupils.Constriction of blood vessels serving the skin and viscera. Increased glycogenolysis in the liver, releasing glucose into the bloodstream. Increased secretion of epinephrine and norepinephrine from the adrenal medulla, further enhances the effects listed above.

Explanation: Sympathetic stimulation is the part of the autonomic nervous system that is responsible for the “fight or flight” response. This is a reaction to a stressor that prepares the body for action. The sympathetic nervous system is activated when a person is faced with a stressful situation.

It is responsible for many physiological changes that help the body respond to stress. The sympathetic nervous system activates the adrenal medulla, which secretes epinephrine and norepinephrine. These hormones enhance the effects of sympathetic stimulation.

They increase heart rate and blood pressure, increase blood flow to skeletal muscles, and decrease blood flow to the digestive system. They also cause glycogenolysis in the liver, which releases glucose into the bloodstream.

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Dorado
Voo
What does the image above represent?
dichotomous key
cladogram
family tree
Obinomial nomenclature

DoradoVooWhat does the image above represent?dichotomous keycladogramfamily treeObinomial nomenclature

Answers

Answer:

B. cladogram

Explanation:

"A cladogram is a diagram used to represent a hypothetical relationship between groups of animals, called a phylogeny. A cladogram is used by a scientist studying phylogenetic systematics to visualize the groups of organisms being compared, how they are related, and their most common ancestors."

The image above represents a cladogram.

A cladogram is an evolutionary tree that diagrams the traditional connections among organisms.

What is a cladogram?

A cladogram is a type of chart that shows theoretical relationships between classes of organisms.

A cladogram compares a tree, with branches off the main trunk. Key elements of a cladogram are the root, clades, and nodes. The root is an initial progenitor that is familiar to all groups branching off from it.

Thus, a cladogram is an evolutionary tree that diagrams the traditional connections among organisms.

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19.
Which organic molecules are nucleic acids?

enzymes and proteins

DNA and RNA

glycogen and ATP

lipids and carbohydrates

Answers

Answer:

DNA and RNA

Explanation:

DNA- Deoxyribonucleic acid

RNA- Ribonucleic acid

for biologists studying a large flatworm population in the lab, which hardy-weinberg condition is most difficult to meet? for biologists studying a large flatworm population in the lab, which hardy-weinberg condition is most difficult to meet? no genetic drift no selection no gene flow no mutation

Answers

The Hardy-Weinberg condition that is most difficult to meet is most likely the no mutation condition.

The Hardy-Weinberg principle states that the frequencies of alleles and genotypes in a population will remain constant from generation to generation in the absence of evolutionary forces such as genetic drift, selection, gene flow, and mutation. In a laboratory setting where the population is controlled and isolated, the other three conditions (no genetic drift, no selection, and no gene flow) can be relatively easily met.

However, mutations are spontaneous changes in the genetic material that can introduce new alleles into a population. Since mutations occur randomly, they can disrupt the equilibrium predicted by the Hardy-Weinberg principle. Thus, it is challenging to prevent mutations from occurring in a laboratory setting, making it difficult to meet the "no mutation" condition.

Hence, the correct option is D.

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Which would have a bigger effect on an organism, an error during transcription or a point mutation?

Answers

Answer: Point mutation would have a bigger effect on an organism.

Explanation:

mary was preparing to exercise and her heart rate went from 56 to 84 beats per minute. most of this effect can be attributed to…

Answers

The significant increase in Mary's heart rate from 56 to 84 beats per minute during exercise can mainly be attributed to physiological factors such as increased oxygen demand and metabolic activity.

During exercise, the body's oxygen demand rises to meet the increased metabolic needs of the muscles. To deliver oxygen and nutrients efficiently, the heart pumps blood at a faster rate. The increase in heart rate allows for more blood to be circulated per minute, ensuring an adequate supply of oxygen and nutrients to the working muscles. This elevated heart rate is a result of the body's natural response to physical activity.

Additionally, exercise stimulates the sympathetic nervous system, which releases stress hormones like adrenaline. Adrenaline enhances heart rate by increasing the electrical conduction within the heart and boosting the strength of contractions. These factors, combined with the body's need for increased oxygen and metabolic activity, contribute to Mary's heart rate rising from 56 to 84 beats per minute during exercise.

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what modification neutralizes the charges on histones that promote ionic interaction with dna?

Answers

The modification that neutralizes the charges on histones that promotes ionic interaction with DNA is called acetylation.

Acetylation is the method of transferring an acetyl group from one molecule to another. In cells, histone acetylation is a chemical modification that occurs on lysine residues in histone proteins, resulting in the neutralization of the charges that promote ionic interaction with DNA.Acetylation is performed by histone acetyltransferases, which add acetyl groups to lysine residues on histone tails, and the acetyl groups neutralize the positive charges on the lysine residues, resulting in a decrease in histone-DNA interaction. This process alters the chromatin structure, allowing DNA transcription and, ultimately, gene expression.

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Samples for a compound light microscope are typically prepared on _______.
a.
glass slides
b.
coverslips
c.
a portion
d.
dissecting trays


Please select the best answer from the choices provided

A
B
C
D

Answers

A, I think cuz we used glass slides in my high school for microscopes.

a defensive mechanism observed in some sea cucumbers that involves the sudden expulsion of the gut and other internal organs through the mouth or anus is known as .

Answers

Evisceration is a defense strategy used by certain sea cucumbers that involve the abrupt evacuation of the gut and other internal organs through the mouth or anus.

The defensive mechanism observed in some sea cucumbers is known as evisceration. This is an adaptive survival strategy that helps the sea cucumber escape from predators or other dangers.

The mechanism observed in some sea cucumbers is called evisceration. This is a defense mechanism used by the sea cucumber to distract predators or remove parasites from its body.

When threatened, the sea cucumber will quickly expel its gut and other internal organs through its mouth or anus, effectively making itself less appetizing to the predator. The expelled organs can be regenerated over time.

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The term used to describe a location with many different cultural groups is
A. culturally diverse
B. globalization
C. multiple perspectives
D. ethnicity

Answers

A
It’s the only answer that even makes sense if you really think about it.

que es un nucleótido??

Answers

Explanation:

Nucleotides are organic molecules consisting of a nucleoside and a phosphate. They serve as monomeric units of the nucleic acid polymers deoxyribonucleic acid and ribonucleic acid, both of which are essential biomolecules within all life-forms on Earth

according to the megaplate video, why does bacterial growth pause when the bacteria reach an antibiotic boundary?. choose the most accurate response.

Answers

Antibiotics are not present at either end of the MEGA plate. However, the scientists paint bands of antibiotics of increasing potency every few centimeters in toward the center. Every increase in antibiotic concentration is a stressor that the bacteria (which are simply E. coli) must deal with.

In the MEGA plate video, it''s both beautiful and terrifying to see antibiotics fall victim to the might of evolution. The bacteria (shown in milky white) are all stopped at the first antibiotic band.

However, a small group of bacteria with a mutation that allows them to survive the chemical attack manages to break through. These mutant bacteria become the population's dominant type. This process is repeated for each stronger antibiotic band until the bacteria achieve total resistance at the center.

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A client with a pathophysiologic condition that affects the desmosomes is most likely to exhibit?

Answers

A  client with pathophysiology condtions that affects the Desmosomes is most likely to exhibit seperation at the junctions between epithelial cells.

Where are Desmosomes function?

Desmosomes are intercellular junctions that confer strong cell to cell adhesion.They are found in epithelia and cardiac muscle and are located at the cell membrane,where they act as anchors for intermediate filaments.

Desmosomes represents major intercellular adhesive junctions at basolateral membranes of epithelial cells and in other tissues.

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what is the caloric value of a carbohydrate

Answers

Answer:

4 kcal per gram of carbohydrate

Answer:

4 kcal per gram of carbohydrate

Explanation:

A bacterial species differs from a species of eukaryotic organisms in that a bacterial species.

Answers

The way in which a bacterial species differs from a eukaryotic species is because bacteria is a population of cells with similar characteristics.

Bacteria- Microorganisms that are unicellular, capable of autonomous reproduction, and generally free-living are known as bacteria. In nature, bacteria are found everywhere. The foundation of all life on earth, they are architecturally basic but functionally sophisticated creatures.

Eukaryotic Organism- Every creature or cell with an easily identifiable nucleus. The nucleus of a eukaryotic cell, which houses the very well chromosomes (bodies holding the genetic material), is surrounded by a nuclear membrane. For Example, Epithelial cell, WBC, yeast.

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Match each description

Match each description

Answers

The answer is as follows:

SolidGasBose-Einstein condensate liquidPlasmaWhat are Bose-Einstein condensate and plasma?

The key contrast between plasma and Bose-Einstein condensate is that the plasma condition prevents a gas of ions and free electrons. In comparison, Bose-Einstein condensate collects a gas of bosons at low viscosity, which is cooled to a low temperature close to final zero.

Thus, this could be the answer.

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The rigid vibrating particles are found in solid, while the stripped electrons of particles at higher temperatures are found in gas.

What are Bose-Einstein condensate and plasma?

The key contrast between plasma and Bose-Einstein condensate is that the plasma condition prevents a gas of ions and free electrons, whereas Bose-Einstein condensate collects a gas of bosons at low viscosity, which is cooled to a low temperature close to final zero.

The rigid vibrating particles are found in solid.

Stripped electrons at high temperatures are found with particles of Gas.

Particles acting as a single particle at low temperatures are of Bose-Einstein Condensate.

Independently moving particles are found with Liquid.

The relative movement of the particle without being completely separated is plasma.

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Javier tells Ben that he wants to get a new sports car. Ben immediately understands why, because he is familiar with the common characteristics of sports cars and knows what makes them different from family cars. Ben is using mental categories called __________.

Answers

Javier tells Ben that he wants to get a new sports car. Ben immediately understands why, because he is familiar with the common characteristics of sports cars and knows what makes them different from family cars. Ben is using mental categories called prototypes.

Ben is using mental categories called prototypes to understand Javier's desire to get a new sports car. Prototypes are cognitive representations of typical or ideal examples within a category. Ben's familiarity with the common characteristics of sports cars allows him to quickly recognize and categorize Javier's desire based on those prototypes.

Prototypes serve as mental reference points that help individuals make sense of the world and organize information. They are formed through experience and exposure to exemplars within a category. In this case, Ben's knowledge of sports cars and their distinguishing features enables him to recognize Javier's interest in a new sports car based on the prototype he has in mind.

Understanding prototypes allows individuals to make inferences, predictions, and generalizations based on the shared characteristics of a category. It facilitates efficient information processing and cognitive shortcuts in recognizing and categorizing objects, events, or concepts.

Ben's use of mental categories called prototypes allows him to quickly comprehend Javier's desire for a new sports car based on his knowledge of the common characteristics associated with sports cars. Prototypes play a crucial role in cognitive processes such as categorization, inference-making, and generalization, helping individuals organize and understand the world around them.

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Which two processes during cellular respiration release chemical energy
stored in chemical bonds?
A. The conversion of NADH to NAD*
B. The splitting of glucose
C. The production of ATP by ATP synthase
D. The conversion of FAD to FADH2

Answers

The splitting of glucose  release chemical energy stored in chemical bonds.

In process of cellular respiration, the energy in food is converted into the energy that is used by the body's cells. In process of cellular respiration, glucose and oxygen are converted into carbon dioxide and water, and the energy is converted to ATP.

Cellular respiration, is the process that takes place in the mitochondria of organisms which break down sugar in the presence of oxygen and release energy in form of ATP. Carbon dioxide and water are released as  the waste products.

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what would the probability be that the son has dark hair?

what would the probability be that the son has dark hair?

Answers

There’s a 50/50 chance for the son to either have blond or dark hair
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