which technique cannot be used to analyze gene expression?

Answers

Answer 1

Answer:

So the mnemonic that I know is SNOW DROP. Southern=DNA, Northern=RNA, O=O, and Western=Protein. The question asks, "Which technique CANNOT be used to analyze gene expression?" and the answer is Southern because it is a "techniue used to detect a particular sequence in a sample of DNA."

Answer 2

PCR that is polymerase chain reaction techique is a  technique cannot be used to analyze gene expression.

PCR is a very sensitive technique that allows rapid amplification of a specific segment of DNA.PCR makes billions of copies of a specific DNA fragment or gene, which allows detection and identification of gene sequences using visual techniques based on size and charge.Modified versions of PCR have allowed quantitative measurements of gene expression with techniques called real-time PCR.

The technique of PCR  is used for the purpose of amplification of DNA and thus cannot be used to analyze gene expression.

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Related Questions

Sugar is food for plants, what is the type of sugar plants make?

Answers

I think you are looking for the answer glucose.

Plants make glucose, a type of simple sugar.

Which environmental change occurs most rapidly?

Answers

Answer:

volcanic eruption

Explanation:

"The environmental change that occurs faster is definitely volcanic eruption. It may happen in an instant and destroy everything in its way, whereas the process of regrowth of forest after volcanic eruption is a slow one."

Answer:

Abrupt climate change.

Explanation:

Abrupt climate change is climate change that occurs relatively rapidly. Our understanding of past abrupt climate changes and their causes is still in its infancy; most of the research on this topic has occurred since the early 1990s.

different cells make and accumulate different sets of rna and protein molecules, despite all sharing the same dna. this process is known as:

Answers

Despite having the same DNA, distinct cells produce and accumulate unique sets of rna and protein molecules due differentiation.

It is known that cells specialise through a process called cell differentiation. Pluripotency, which can be shown in stem or progenitor cells at the start of the differentiation process, refers to the capacity to develop into any form of cell. Even though all the cells in a multicellular organism have the same DNA, they may express quite diverse gene sets. A cell's distinctive characteristics are determined by the set of proteins and functional RNAs that are expressed from its set of expressed genes.

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Different microscopic organisms cause different diseases. When a dangerous microscopic organism is transferred from one person to many people, the disease it carries will spread. Why is this considered a theory instead of a hypothesis or a law? It is a statement of fact about how nature works instead of an explanation. It provides an explanation for many observations and accepted hypotheses. It describes how to test a possible explanation of one specific relationship. It has not changed over time even though new technology has been developed.

Answers

Answer:

The correct answer is - it provides an explanation for many observations and accepted hypotheses.

Explanation:

The theory is well tested or substantiated explanation for the observation of the natural phenomenon, that included or incorporated various laws, hypothesis, or facts.

It is not just a possible explanation but a well-tested and proven explanation with the help of scientific experiments in a scientific method.

Thus, the correct answer is -  it provides an explanation for many observations and accepted hypotheses.

Answer:

Explanation:

the answer is B. for a short answer

Some proteins can bind to other proteins only after phosphorylation. Which bond or force is likely important for binding to occur after, but not before, phosphorylation?.

Answers

Hydrophobic clustering is the main force drives the folding of protein, while the hydrogen bond and ionic bond are responsible for maintaining the stable structure of protein.

The hydrophobic effect refers to a nonpolar molecular surface's energy tendency to connect with other nonpolar molecular surfaces and consequently push water molecules away from the interacting surfaces. When hydrophobic molecules or moieties interact with a hydrophilic environment, such as when oil droplets clump together in water, the phenomenon is known as the hydrophobic effect.Both enthalpic and entropic effects contribute to the hydrophobic effect. Short-range attractive interactions known as hydrophobic interactions play a significant role in the binding affinities between ligands and receptors. Although less geometrically limited than hydrogen-bonding interactions, they nonetheless contribute to specificity.

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2. In horses, the allele for black hair (B) is dominant, and the allele for brown hair (b) is recessive. A horse
breeder crosses a homozygous dominant mother with a heterozygous male. What is the percentage the
offspring will have brown hair?
(write as % and as a fraction)
Draw the Punnett Square and show your work.

Answers

The offspring will have a 25% chance of having brown hair, which can be expressed as a fraction as 1/4.

What are the genotypes of the mother and father? The mother's genotype is BB, meaning she is homozygous dominant for the black hair trait. This means that she has two copies of the dominant allele for black hair. The father's genotype is Bb, meaning he is heterozygous for the black hair trait. He has one copy of the dominant allele for black hair and one copy of the recessive allele for brown hair. The father's phenotype is black hair, because the dominant allele masks the recessive allele. The mother's phenotype is also black hair, because she is homozygous dominant for this trait. The offspring of this mating will have a 50/50 chance of having black hair (homozygous dominant) or brown hair (heterozygous). This is because each parent has contributed one allele for black hair, and the offspring will receive one allele from the mother and one from the father.

Punnett Square:

BB   Bb

Bb   bb

Percentage: 25% or 1/4

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if someone says hola to you, would you say como estas or hola back?

Answers

Answer:

you can say either of them, but most people usually just say hola and then that's when they say como estas

Explanation:

Answer:

You always greet them first and then ask them how their day is. :)

The thermal energy of an object decreases. Which measurement of the object will change?

Answers

Answer:

temperature

(and kinetic energy ? ? maybe)

let me know if its right

Explanation:

True or False:
Evolution acts on life and leads to adaptations in species.

Answers

True. In biology, evolution is the change in the characteristics of a species over several generations and relies on the process of natural selection.
True or False:Evolution acts on life and leads to adaptations in species.

why is a green leaf used in the experiment​

Answers

Answer:

to know whether there is starch in the leaf

which can influence sex determination? check all that apply. a. the X chromosome
b. the Y chromosome c. the temperature at which eggs are incubated d. the pH at which eggs are incubated e. the length of time during which eggs are incubated

Answers

Sex determination can be influenced by several factors that are a. the X chromosome, b. the Y chromosome, c. the temperature at which eggs are incubated, d. the pH at which eggs are incubated, and  e. the length of time during which eggs are incubated

The X chromosome and Y chromosome play a crucial role in determining the sex of an organism. Typically, an individual with XX chromosomes will develop as a female, while an individual with XY chromosomes will develop as a male. However, there are some exceptions to this rule. For example, in birds, reptiles, and some fish, the temperature at which eggs are incubated can influence sex determination, this is known as temperature-dependent sex determination.

The pH at which eggs are incubated can also affect sex determination in some species. Additionally, the length of time during which eggs are incubated can impact sex determination in certain organisms. Overall, the factors that influence sex determination can vary depending on the species and can involve genetic, environmental, or a combination of both factors. So therefore the correct answer is all above.

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a_fault is from the hanging wall drops down

Answers

Answer:

If the hanging wall moves down relative to the footwall, the fault is a normal fault. Normal faults are caused by tensional stress, or stress that pulls rocks apart

Explanation:

Which organelle is present in a prokaryotic organism, but in a primitive form?

Answers

The major differences between Prokaryotic and Eukaryotic cells are that prokaryotes do not have a nucleus as a distinct organelle and rarely have any membrane bound organelles [mitochondria, chloroplasts, endoplasmic reticulum, golgi apparatus, a cytoskeleton of microtubules and microfilaments] (the only exception may

What is the primary function of the Cell Wall?
1.Take in carbon dioxide
2.builds proteins
3.to protect and provide support for the cell
4.convert solar energy to chemical change

What is the primary function of the Cell Wall?1.Take in carbon dioxide2.builds proteins3.to protect and

Answers

to protect and provide support for the cell

All life-forms survive on resources from the environment. So, each ecosystem can support only a certain number of organisms of each species. What would most likely happen if a species of herbivores exceeded what the particular ecosystem could support?

A. There will be less food for the secondary consumers.
B. There will be too few producers to provide energy for the system.
C. There will be no decomposition by bacteria and fungi.
D. There will be more food for the other primary consumers.

Answers

Answer:

maybe D

Explanation:

Answer: it's the second one

Explanation:

if there are lots of animals who eat grass then there will be very few producers (which are those plants + grass). Hope this helps!

hope this helps

people with sickle-cell disease have 1. defective phenylalanine hydrolyase. 2. a defective oncogene. 3. abnormal cholesterol transport. 4. defective hemoglobin. 5. an abnormality in their dna replication

Answers

People with sickle-cell disease have (4) defective hemoglobin.

Sickle cell anemia is the genetic disorder where the shape of the hemoglobin changes from the usual concave into a sickle-like. This happens due to the change in one amino acid in the DNA sequence. The disease is an autosomal recessive condition.

Hemoglobin is the red-colored pigment found in the blood of individuals. It is concave in shape and also contains the iron element. The main function of hemoglobin apart from providing color is to transport oxygen across the whole body. One hemoglobin molecule can bind to 4 oxygen atoms at once.

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which is the main function of the respiratory system?
a. To protect the body from illness.
b. To push blood to all parts of the body
c. To protect the internal organs
d. To help oxygen get into blood vessels

Answers

D. To help oxygen get into blood vessels



this answer i need pls ill mark brainliest

this answer i need pls ill mark brainliest

Answers

All - growth, exchange of gases, excretion, reproduction, and death (option d).

All living organisms undergo various life processes to maintain their existence. Let's analyze each option to determine which life processes are carried out by an organism's cells:

A. Only growth and exchange of gases: While cells are involved in growth and exchange of gases, they also participate in other life processes. This option is incomplete.

B. Only growth, exchange of gases, and reproduction: Cells play a crucial role in reproduction as they are responsible for the production of gametes and the process of cell division. However, there are additional life processes that cells also undertake.

C. Only growth, exchange of gases, excretion, and reproduction: This option includes excretion in addition to growth, exchange of gases, and reproduction. Cells participate in excretion by eliminating waste materials. However, there is one more life process that cells experience.

D. All - growth, exchange of gases, excretion, reproduction, and death: This option encompasses all the mentioned life processes. Cells are involved in growth as they undergo cell division and increase in number. They exchange gases through processes like respiration. Cells excrete waste products. They participate in reproduction through the formation of gametes and cell division. Lastly, cells also experience death as they have a limited lifespan.

Therefore, the correct answer is D. All - growth, exchange of gases, excretion, reproduction, and death.

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What are the properties of seismic waves, sound waves, and the different types of electromagnetic waves?

Answers

Answer:

they have different frequencies

Explanation:

All electromagnetic waves have various same properties like reflection, refraction, interference, and diffraction.

What are electromagnetic waves?

The oscillations between an electric field and a magnetic field produce waves known as electromagnetic waves or EM waves. In other words, magnetic and electric fields oscillate to form electromagnetic (EM) waves. An electromagnetic wave's magnetic and electric fields are parallel to one another and to the wave's direction.

Electromagnetic waves include radio waves, television waves, microwaves, sound waves, seismic waves, and infrared waves. The only thing separating them is the wavelength. The distance between each wave's peak and the next is called the wavelength.

They have properties of reflection, refraction, diffraction, and interference.

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What are the 3 main arm muscles?

Answers

Biceps brachii:

Brachialile

Coracobrachialis:

This same triceps brachii, biceps brachii, brachialis, as well as brachioradialis are now the muscles anywhere along humerus help move this same forearm.

Brachialis: What is it?

The intermediate anterior forearm serves as the origin of the brachialis, a shoulder flexor that implants onto ulnar anterior surface. One of the biggest elbow flexors, muscular brachialis, allows for complete flex of the forearms somewhere at elbow.

What functions does the brachialis perform?

The elbow's powerhouse, the force is also known, is well known. It is a significant forearm flexor at the rotator cuff, flexing the elbow in all directions. The first and only pure abductor of the rotator cuff that exerts the most force throughout elbow flexion is really the brachialis.

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Matter is anything that takes up space and has
Answer here I
SUBMIT

Answers

Answer:

If I am not mistaken, it should be: Matter is anything that takes up space and has mass.

Explanation:

:)

What are the disorder caused by mutation?

Answers

The disorders caused by mutations are called genetic disorders.

What are genetic mutations?

Genetic mutations are alterations in the genetic material of an organism due to changes in the linear order of nucleotide bases in the genome that form the genetic material of the cells of such organism.

Moreover,  genetic mutations cause genetic disorders that are inherited through the germinal line when these mutations are able to pass from parents to offspring, in a process that involves the transference of genetic material from the egg cell or the sperm cell to the first somatic cell which is called the zygote.

Therefore, with this data, we can see that  genetic mutations are able to cause genetic disorders that are alterations in the genetic material passed from parents to offspring through the germinal line, i.e. by the egg cell or the spermatic gametic cells

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Why do you think mapping the bedbug genome may help scientists control pests?

Answers

Explanation:

When mapping the genome, it shows where other pests are more likely to appear. This means more control over the rate of pest growth, and they can stop reproduction from increasing.

Understanding the genome will enable scientists to know what traits the bug has and whether they have any resistance to insecticides. The bugs may have natural resistance to certain insecticides, but not to others. This research may help scientists develop effective insecticides.

Peptidoglycan is the material found in the bacterial____
that forms a molecular basis of the Gram stain.
_organelles
_cell wall
_nucleus

Answers

Answer:

The Cell Wall of Bacteria

Bacteria are protected by a rigid cell wall composed of peptidoglycans.

LEARNING OBJECTIVES

Recall the characteristics of a bacterial cell wall

KEY TAKEAWAYS

Key Points

A cell wall is a layer located outside the cell membrane found in plants, fungi, bacteria, algae, and archaea.

A peptidoglycan cell wall composed of disaccharides and amino acids gives bacteria structural support.

The bacterial cell wall is often a target for antibiotic treatment.

Key Terms

binary fission: The process whereby a cell divides asexually to produce two daughter cells.

image

Bacterial Cell Wall: The anatomy of bacterial cell structure.

Bacterial cells lack a membrane bound nucleus. Their genetic material is naked within the cytoplasm. Ribosomes are their only type of organelle. The term “nucleoid” refers to the region of the cytoplasm where chromosomal DNA is located, usually a singular, circular chromosome. Bacteria are usually single-celled, except when they exist in colonies. These ancestral cells reproduce by means of binary fission, duplicating their genetic material and then essentially splitting to form two daughter cells identical to the parent. A wall located outside the cell membrane provides the cell support, and protection against mechanical stress or damage from osmotic rupture and lysis. The major component of the bacterial cell wall is peptidoglycan or murein. This rigid structure of peptidoglycan, specific only to prokaryotes, gives the cell shape and surrounds the cytoplasmic membrane. Peptidoglycan is a huge polymer of disaccharides (glycan) cross-linked by short chains of identical amino acids (peptides) monomers. The backbone of the peptidoglycan molecule is composed of two derivatives of glucose: N-acetylglucosamine (NAG) and N-acetlymuramic acid (NAM) with a pentapeptide coming off NAM and varying slightly among bacteria. The NAG and NAM strands are synthesized in the cytosol of the bacteria. They are connected by inter-peptide bridges. They are transported across the cytoplasmic membrane by a carrier molecule called bactoprenol. From the peptidoglycan inwards all bacterial cells are very similar. Going further out, the bacterial world divides into two major classes: Gram positive (Gram +) and Gram negative (Gram -). The cell wall provides important ligands for adherence and receptor sites for viruses or antibiotics.

THANKS ME LATER IF YOU IF YOU LIKE IT

That is a big answer. ^ but yeah its cell wall.

The lac operon in E.coli regulates genes that code for enzymes required for the breakdown of lactose. The lac operon is
operon that is activated in the presence of

Answers

The lac operon in E.coli regulates genes that code for enzymes required for the breakdown of lactose. The lac operon is an inducible operon that is activated in the presence of lactose.

It is a cluster of genes in bacteria that is responsible for the metabolism of lactose. The lactose operon consists of three structural genes, lac Z, lac Y, and lac A, that code for enzymes that break down lactose, and a regulatory region, which includes the promoter, operator, and lac I gene. It functions as a switch that turns genes on or off in response to environmental changes. The regulatory region of the lactose operon contains the operator and the promoter. The operator is a DNA sequence that regulates the expression of the genes by acting as a binding site for the regulatory protein.

The promoter is a DNA sequence that is recognized by RNA polymerase, which initiates the transcription of the genes. The lac I gene codes for a regulatory protein called the lac repressor. The lac repressor binds to the operator region of the lac operon and prevents RNA polymerase from transcribing the genes. In the absence of lactose, the lac repressor is active and the lac operon is turned off.

When lactose is present in the environment, it binds to the lac repressor and changes its conformation, preventing it from binding to the operator. This allows RNA polymerase to bind to the promoter and transcribe the genes necessary for lactose metabolism. Thus, the lactose operon is an inducible operon that is activated in the presence of lactose.

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Which substance is associated with immunosuppression in shock? A. Glycogen B. Cortisol C. Renin D. Antidiuretic hormone.

Answers

D. Antidiuretic hormone.

Cortisol is a substance is associated with immunosuppression in shock. Option (B) is correct.

Cortisol is a hormone produced by the adrenal glands in response to stress. During shock, the body undergoes a significant stress response,

leading to the release of cortisol. Cortisol plays a crucial role in regulating the immune system. However, in high levels, it can have immunosuppressive effects.

During shock, cortisol levels rise as part of the body's stress response. High levels of cortisol can suppress the immune system, inhibiting immune cell function and reducing the body's ability to mount an effective immune response. This immunosuppression can leave the individual more susceptible to infections and impair the body's ability to fight against pathogens.

It's important to note that while cortisol has important regulatory functions in the body, excessive or prolonged elevation due to conditions like shock can have negative effects on the immune system.

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This is for my biology class. It is for the osmosis potato lab (Google it if you don't know what it is) and I have to answer this question. I also have to include the words at the bottom. This is really confusing and I would be so happy if someone could please help me with this!

How can a scientist use this lab to determine the amount of solute in an Idaho potato?

Use the following vocabulary words in your final conclusion statement below:
Hypertonic, hypotonic, isotonic, dynamic equilibrium, solute, solvent, osmosis

Answers

Explanation:

A scientist can use this experiment to determine the amount of solute in any potato.

hypotonic solution is a relative term. it means when we compare two solutions one of them is considered to be a hypotonic if it's solute concentration is less than the other.

so if the solution has less solute concentration its hypotonic solution and if it has more amount of solute its hypotonic solution. if both solutions have same amount of solute then it will be considered as a isotonic solution. when we separate two solution hypertonic and hypotonic by a semipermeable membrane there occurs diffusion of water molecules from its higher concentration to its lower concentration that is from hypotonic solution to hypertonic solution.

this movement of water molecules is called osmosis. this occurs in order to obtain an dynamic equilibrium between the 2 solutions.

here we take start as solute and water as solvent.

thus when a scientist and potato in a hypotonic solution the water moves inside the potato cavity. the more the transfer of water molecules in the potato cavity the more in the sugar quantity present in the potato. this is because the water will try to obtain a dynamic equilibrium between the two solutions if the sugar quantity in the potato cavity is high then the water molecules needed to dilute that solution will also be high.

in in this way a scientist can determine the amount of salt present in the potato.

( this is another thing I need help with )
What is the main characteristic of gymnosperm seeds?
A. They are flowers
B. They are born in cones
C. They are fruits
D. they germinate

Answers

Answer:

D

Explanation:

Because the gymnosperm germinates

which of the following organisms has the simplest cellular structure? a. bacterium b. earthworm c. mushroom d. sunflower

Answers

Bacteria will have the simplest cellular structure. So the correct option is A.

What are prokaryotic cells?

A cell type without a genuine nucleus or membrane-bound organelles is referred to as prokaryotic. All the other types of life are eukaryotic, whereas organisms in the categories of Bacteria and Archaea are centered on the prokaryotic cell.

Prokaryotic species, on the other hand, are exceedingly prevalent and account for a large portion of the biomass on Earth.

Unicellular organisms are those that have prokaryotic cells. We refer to them as "prokaryotes." A prokaryotic cell may operate as a live creature thanks to a number of factors. Prokaryotes have a cell membrane covering them at first.

With the help of this membrane, they may engineer a particular environment for metabolic processes within the cytosol. Second, these cells contain both ribosomes and free DNA.

Even though ribosomes are organelles, a plasma membrane does not enclose them.

Therefore the correct option is A.

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A lake such as the one
pictured is considered an
open system because

A. it is isolated by the mountains
surrounding it.
B. there is an input and output of energy
and water.
C. there are no interactions with other
bodies of water.

Answers

Answer: B.

Explanation: Open systems are defined by their interaction with external forces therefore B is correct

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